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KMID : 1134820070360081077
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Journal of the Korean Society of Food Science and Nutrition
2007 Volume.36 No. 8 p.1077 ~ p.1082
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Characteristics of Pichia anomala K15 Producing Killer Toxin Isolated from Traditional Nuruk
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Jung Hee-Kyoung
Park Chi-Duck
Lee Ki-Dong
Park Seung-Chun
Prak Hwan-Hee
Hong Joo-Heon
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Abstract
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In this study, killer yeasts were isolated from traditional Nuruk to improve storage and suppress contaminant in food industry. Among killer yeasts, yeast K15 showed strong killer toxin activity and inhibited growth of Salmonella Typhimurium and Vibrio parahaemolyticus. Killer yeast K15 was identified with Pichia anomala by the Microlog TM 4.0 identification system and homology of the ITS sequence. Killer toxin generated from P. anomala K15 was inactivated by pronase E and suggested to be a protein. Therefore killer toxin of P. anomala K15 was thought to be safe in human such as bacteriocin. P. anomala K15 was sufficient for growth in 50% glucose and could be used to prevent contaminant in initial stages of alcohol beverage fermentation.
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KEYWORD
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Nuruk, killer toxin, Pichia anomala K15, isolation
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